How to reconstitute TB-500 powder with bacteriostatic water: run it down the vial wall, swirl gently, verify the concentration. Educational only.
Pre-injection checklist
- ✓Research-context note: TB-500 is not approved by the FDA for human use, and this page describes reconstitution technique for educational and research purposes only. Nothing here recommends using any compound, and it is not medical advice.
- ✓Read the vial label for the peptide mass in milligrams, commonly 2 mg, 5 mg, or 10 mg. That number and the volume of water you add together fix the final concentration.
- ✓Decide the diluent volume before you start. This page explains how volume sets concentration; it does not tell you a dose or a volume to use.
- ✓Let the vial and the bacteriostatic water reach room temperature, then lay everything out on the clean surface.
- ✓Wipe both rubber stoppers with an alcohol pad and let them air-dry fully before the needle touches them.
Step-by-step guide
- 1
Choose and draw the diluent volume
Decide how much bacteriostatic water you will add, then draw exactly that volume into the mixing syringe. The volume is what fixes the concentration: 5 mg of powder in 2 mL of water gives 2.5 mg/mL, while the same 5 mg in 1 mL gives 5 mg/mL.
Pro tipAlways check the fluid is clear and colourless before injection. - 2
Run the water down the glass wall
Insert the needle through the peptide vial stopper and let the water trickle slowly down the inside wall of the glass. Do not jet it straight onto the powder pellet; a gentle stream is easier on the fragile peptide.
Pro tipAlways check the fluid is clear and colourless before injection. - 3
Swirl, never shake
Roll or swirl the vial gently in a slow circle until the powder fully dissolves. TB-500 goes into solution readily, and swirling avoids the foam that shaking creates, which peptide-handling references describe as a source of degradation.
Pro tipAlways check the fluid is clear and colourless before injection. - 4
Inspect the finished solution
A correctly reconstituted solution is clear and free of particles. Do not use a solution that stays cloudy, shows floating flakes, or is discolored; our cloudy-peptide troubleshooting guide covers what those signs mean.
Pro tipAlways check the fluid is clear and colourless before injection. - 5
Label, date, and refrigerate
Write the reconstitution date and a beyond-use date on the vial and store it at 2 to 8 °C. Reconstituted peptide is far less stable than the dry powder; our guide on how long reconstituted peptide lasts explains the timelines reported in the literature.
Pro tipAlways check the fluid is clear and colourless before injection.
After the injection
- Store the reconstituted vial in the refrigerator at 2 to 8 °C, away from the freezer compartment and the door.
- Use a fresh, sterile needle for every withdrawal, not the mixing needle.
- Drop the mixing needle straight into the sharps container without recapping it.
Common mistakes
- The powder will not dissolve after gentle swirling, or particles or cloudiness remain in the solution.
- The solution is discolored or has visible flakes floating in it; do not use it.
- Any uncertainty about concentration or the handling of a research compound; stop and consult a qualified professional. This page does not authorize use of any compound.
Frequently asked questions
How do I reconstitute TB-500?+
Draw up the volume of bacteriostatic water you have decided on, then let it run slowly down the inside wall of the powder vial. Swirl gently until everything dissolves, inspect the clear solution, and label the vial with the reconstitution date and a beyond-use date. This page explains the technique in a research context, not a dose.
How much bacteriostatic water do I add to TB-500?+
There is no single answer, because the volume is what sets the concentration. A 5 mg vial in 2 mL of water is 2.5 mg/mL, and the same vial in 1 mL is 5 mg/mL. Choose the volume deliberately, record it, and use our peptide calculator to see how volume, concentration, and syringe units relate.
Can I use sterile water instead of bacteriostatic water for TB-500?+
The two differ by preservative. Bacteriostatic water contains 0.9% benzyl alcohol, which is why it is described for multi-dose vials that are entered more than once. Sterile water has no preservative and is described for single use. Peptide-handling references generally use bacteriostatic water for a vial you will draw from repeatedly.
How long does reconstituted TB-500 last?+
Reconstituted peptide is far less stable than the dry powder and should be kept refrigerated at 2 to 8 °C. Reported beyond-use windows vary by compound and storage in the stability literature, so honor the beyond-use date you write on the vial. Our guide on how long reconstituted peptide lasts covers the reported timelines.
Is TB-500 approved for human use?+
No. TB-500 is a research peptide that is not approved by the FDA for human use, and the FDA has placed certain peptides on its bulk-substance lists under review. This page describes reconstitution technique for educational and research purposes only, and it does not recommend using any compound.